Part 1 Principles |
Confocal Microscopy tutorialPart 2 application of confocal microscopy4. Thick specimenAs emphasized in previous section, one forte of confocal microscopy is its power to resolve thick specimen thanks to its depth discrimination property. It can be used for tissue block, small organ, embryo, etc. The following two images are taken from the same field of the plant of 60 µm thick by different means. The first one is taken by CCD camera in the wild field microscope configuration. the second one is taken by confocal microscopy with pinhole size at one Airy unit. The difference on the results is striking. It is worth to mention that the difference is not just the sharpness or crisp of the images, but also the ability of resolving the internal structures. Many fine structures clearly visible in confocal image are obscured or totally invisible in the image taken under wild field microscope. For even thicker specimen, no satisfied image can be obtained at all.
This page was last updated
23.03.2004 |