Advanced Microscopy unit

Department of Pathology

Haartman Institute, University of Helsinki

Facilities in AMU

Electron Microscopy:    

JEOL 1400 Transmission Electron Microscope

Location: Room DK 139, Haartman Institute

Accelerate Voltage      40-120 KV Resolution      0.38 nm (point)      0.2 nm   (lattice) Magnification      50 x - 1.2 million x Digital Camera      Olympus-SIS Morada      36 x 24 mm CCD      11 Mega pixels, pixel size: 9 µm      Cooling: Peltier plus water Film Camera       Installed Stage       full motorized: x, y and z and tilt-x. Tilting range      + - 70 degree with high tilting holder      High tilting holder: equipped

JEOL 1200 EX Transmission electron microscope

Location: Room EK 247, Haartman Institute

	Acceleration Voltage     40-120 KV Resolution     0.35 nm (point)     0.16 nm (lattice) Magnification    50 x -300 000 x Digital Camera      Olympus-SIS MegaView G2      1,4 Mega pixels      Pixel size: 6.45 µm       Effective pixel size: Film Camera      Installed Stage: manual Tilting range: + - 60 degree, tilt-x motorized.

JEOL 100S Transmission Electron Microscope

Location: Room EK 247, Haartman Institute

Acceleration Voltage     40-1o0 KV Resolution     0.5 nm (point)     0.34 nm (lattice) Magnification    100 x - 200 000 x Camera     Film Camera Stage: manual Tilting range: no

Auxiliary facilities for electron microcopy

Location: Room EK 247, Haartman Institute

Balzer MED 020 multiple function, modular coating device

For high resolution coating of EM specimen and grids supporting film.
When different modules (chambers and top flanges) are used like showing below, it can be used as different function.

Carbon Coating: thread and E-gun rod coating. Sputtering coating: Gold, Platinum, Tungsten, Chromium E-gun metal coating: Platinum, Tungsten, Titanium, Chromium        Rotary shadowing        Freeze drying        Glow discharge

Leica EM CDP 030 critical point drier for specimen preparation of both TEM and SEM.

Freeze Fracture device Balzers BAF 400 D for making freeze fracture and simultaneously surface coating with metal, carbon or both.

specimen preparation Lab

Location: Room DK 138, Haartman Institute

For tissue embedding, ultra thin sectioning, staining, etc., a specimen preparation lab is available. Equipped with following devices:

Ultra-microtome

Reichert-Jung ultra microtome for routing ultra thin section	Reichert-Jung ultra microtome with cryo-box for frozen untra-thin sectioning

Leica EMStain for automatic thin section staining

      Next