Table 1. Fluorophore Spectral data and corresponding laser for Excitation (PDF file)

Note:
 1. The percentage in table 1 should not be used as the setting for AOTF percentage (as more than one user asked). They speak totally different things. value in table 1 indicate the percent of fluorophore excited under one laser line. AOTF percentage means the percent of light from laser source delivered to the specimen. Maybe, the low value in table 1 implies you have to use high AOTF percentage and vice versa.
2. The range in parenthesis indicates the 20% boundary on both side, at that wavelength, the intensity drop to 20% of its peak value. Value with * means until there, it has not dropped below 20%, further data is not available.

Special attention at choosing fluorophores for this SP2 confocal microscope setup:
1. For green excitation, due to the Krypton  568 nm line used in stead of the traditional HeNe 546 nm line, Alexa 568 is more suitable than TRITC for this laser: 81.53% vs.51.77%.
2. For Red excitation, Texas Red is not a good choice because we do not have HeNe 590 nm line, Kr 568 excites it only 35% while HeNe 633 excites it at a negligible level. Of course, 35% can still be regarded as effective excitation.

Both TRITC and Texas Red can still be used here, but for best results, if there is no special reason for you to stick on them, please avoid them when you prepare specimen for this confocal microscope.