Advanced Microscopy unit
Department of Pathology
Haartman Institute, University of Helsinki
About this Unit
The Advanced Microscopy Unit is a core facility of
Institute, University of Helsinki, located at Meilahti Bio-medical campus of the
university. The unit serves for research projects in Haartman Institute, Biomedicum,
and occasionally, for groups
from other department within University of Helsinki. The unit also serves for clinical
diagnostic work from Department of Pathology, Department of
Virology HUS hospital and gets support from HUS Lab.
Equipped with electron microscope, laser scanning confocal microscope, image analyzer, devices for Video Enhanced Contrast DIC Microscopy (VEC-DIC), a variety of microscopic techniques can be used in the unit.
Taking advantage of high resolution of Electron microscopy, routing observation of ultra-thin section from resin embedded tissue or cells, immuno-EM based on pre- or post-embedding methods, or on frozen section can be easily done. Whole mount cells with or without immuno-labeling suitable for studying cytoskeleton or cell surface structure is also an established technique in this unit.
Taking advantages of depth discrimination or optical sectioning effects of laser scanning confocal microscopy, tomographic view of the cells or tissue can be taken like you are taking a CT image from a cell or tissue block. 3-D reconstruction from the optically sliced series section can be made and viewed from different angle or animated. Thick specimen does not pose a problem in confocal microscopy. Physiological studies like calcium or other ion concentration monitoring is also possible. Some special fluorescent techniques like FRET, FRAP can be easily applied in confocal microscopy. With multi-detectors equipped, multi-labeled specimen which must be detected simultaneously can be easily resolved in confocal microscopy.
Apart from above-mentioned specific application of confocal microscopy, for ordinary fluorescent application, if problematic fluorescence specimen, such as strong background, strong cross-talking in multi-fluorescent dye labeled specimen, if the specimen can provide enough signal to noise ratio, laser scanning confocal microscopy is the right tool to choose.
Living cell imaging is a field depends heavily on the development of rapid detecting device, powerful computer hardware and software, environment chamber which provides the suitable condition for living cells and for recording image in long time period without severe focus shifting. In this unit, all these are possible: Video enhanced DIC microscopy, fluorescent living cell imaging with sensitive CCD camera, or with high resolution image capturing by confocal microscope.
Digital Image Processing and Analysis is an ever growing field. This unit
have some commercial or free software packages for it. Users are
encouraged to come and try to explore it. Some knowledge about digital image
processing operators is needed to run them efficiently. For more technical details, refer to
Services sections on the site.
This page was last updated 04.12.2009